FIG. 4.

Mass spectrum of the product from the GGR reaction with GGPP. GGPP was reduced with an excess amount of S. acidocaldarius GGR, and the product was treated with phosphatase. The retention time of the GC peak of the resulting alcohol corresponded with that of one of the peaks of authentic phytol isomers. The ion spectrum of the peak was similar to those of the authentic compounds. The ions at m/z 296 and 278 correspond to M⁺ and [M-H₂O]⁺, respectively.