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. 2008 Mar 28;190(11):3923–3929. doi: 10.1128/JB.00082-08

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FIG. 5. — Hydrolysis of the GGR reaction product from GGPP. Radiolabeled GGPP and GGPP reduced with S. acidocaldarius GGR were hydrolyzed by acid or phosphatase treatment and then analyzed by normal-phase TLC developed with 2-propanol-aqueous NH₄OH-H₂O (6/3/1). Lane 1, untreated GGPP; lane 2, acid-treated GGPP; lane 3, phosphatase-treated GGPP; lane 4, untreated GGR product from GGPP; lane 5, acid-treated GGR product from GGPP; lane 6, phosphatase-treated GGR product from GGPP. s.f., solvent front; ori., origin.