FIG. 6.
Determination of the substrate preferences of S. solfataricus prenyltransferases. (A) Radiolabeled phytyl diphosphate (lane 1) or GGPP (lane 2) was used as a substrate for a GGGP synthase reaction with racemic glyceryl-1-phosphate. After the reaction, radiolabeled compounds were extracted with butanol and then treated with phosphatase. The resulting alcohols were analyzed by reversed-phase TLC developed with acetone/H2O (9:1). s.f., solvent front; ori., origin. (B) Nonlabeled phytyl diphosphate (lane 1) or GGPP (lane 2) was used as a substrate for a HexPP synthase reaction with [14C]IPP. After the reaction, radiolabeled compounds were extracted with butanol and then treated with phosphatase. The resulting alcohols were analyzed by reversed-phase TLC developed with acetone/H2O (19:1).