FIG. 2.

creD expression during aerobic growth in different media. Expression was measured using β-galactosidase reporter plasmid pUB6070 (A) and RT-PCR (B). DH5α(pUB6070) was grown aerobically for an entire growth curve (A) or to mid-log phase (OD₆₀₀ = 0.5 to 0.6) (B) in nutrient broth (NB; black squares), in M9 minimal medium containing glucose (GLC; open circles), or with pyruvate (PYR; black triangles) as the sole carbon source. (B) Bands represent the RT-PCR product separated using agarose gel electrophoresis and visualized under UV transillumination. The data are averages of (A) or representative of (B) three individual biological replicates.