FIG. 7.

Effect of CreB and CreB-His overproduction on creD expression. Expression was measured using β-galactosidase reporter plasmid pUB6070. DH5α(pUB6070) (A) or DH5α(ΔcreC)(pUB6070) (B) containing either empty pBAD vector (CONT), pUB6073 (CreB), or pUB6074 (CreB-His) were grown to early log phase (OD₆₀₀ = 0.3) in M9 minimal medium containing glucose, and then 0.2% (wt/vol) arabinose was added (+ARA) to induce CreB or CreB-His production, and the cells were grown for a further 2 h. Alternatively, no arabinose was added (−ARA). Error bars indicate standard deviations from at least three independent experiments.