FIG. 1.

proV mRNA levels in intracellular M. tuberculosis. THP-1 cells (A) or human primary macrophages (B to D) containing M. tuberculosis H37Rv were lysed at various time points postinfection to determine steady-state transcript levels relative to those in bacteria at mid-logarithmic phase in broth culture. Levels were normalized relative to a sigA mRNA internal standard as previously described (21, 37) and reported as determined by the method of reference 24, as described in the text (P < 0.05).