FIG. 2.

In vivo determination of flaG and fliST promoters in P. fluorescens F113. The flaG and fliS genes with their upstream regions were cloned into the pFAJ1700 vector, which contains transcriptional terminators flanking both sides of the insertion site. The constructs were used to complement the swimming motility phenotypes of flaG and fliS mutants, after introduction of the constructs by triparental mating. Swimming assays of organisms on LB medium were performed as previously described (7). (A) F113; (B) F113(pFAJ1700); (C) F113 fliS mutant; (D) F113 fliS mutant (pfliS); (E) F113 flaG mutant; (F) F113 flaG mutant (pflaG). Strains, plasmids, and primers used are described in Table S1 in the supplemental material.