TABLE 3.
Termination efficienciesa
| Region (terminator) | Efficiency (%)
|
|
|---|---|---|
| E. coli | A. actinomycetemcomitans | |
| R1+ (T1) | 98 | 99 |
| R2′ (T2) | 50 | 36 |
| R2+ (T2) | 50 | NCb |
| R3+ (T3) | 69 | 86 |
a
The termination efficiencies were calculated from β-galactosidase assays in which strains with terminator-plus reporter plasmids were compared to those with terminator-minus reporter plasmids.
b
NC, not calculated. β-Galactosidase is more highly expressed in A. actinomycetemcomitans when the R2+ region is compared to the R2− region (see the text and Fig. 5E).