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Mutation of ampG causes an increase in PG monomer release. N. gonorrhoeae strains MS11 (wild type), DG132 (ampG), and DG133 (ampG + ampG+, complemented strain) were labeled metabolically by growth in glucose-deficient medium containing [6-3H]glucosamine. Supernatants were collected after 2.5 h of growth, and released PG fragments were separated by size-exclusion chromatography and detected by scintillation counting.
