TABLE 1.
Strains and plasmids used in this study
| Plasmid or strain | Description | Reference or source |
|---|---|---|
| Plasmids | ||
| pIDN3 | N. gonorrhoeae insertion-duplication plasmid (Ermr) | 22 |
| pKH35 | Gonococcal complementation vector (Camr) derived from pGCC6 | 21 |
| pKH37 | Gonococcal complementation vector (Camr) derived from pGCC6 | 30 |
| pDG017 | 1,341-bp fragment containing amiC in pKH37 (Camr); amiC under lac promoter control | |
| pDG131 | 677-bp 5′ ampG fragment in pIDN3 (Ermr) | This work |
| pDG132 | ampG in-frame deletion construct; 722-bp 3′ ampG fragment in pDG131 (Ermr) | This work |
| pDG133 | ampG complementation; 1,331-bp fragment containing ampG of N. gonorrhoeae under lac promoter control in pKH37 (Camr) | This work |
| pDG140 | 1,892-bp ampD region in pIDN3 (Ermr) | This work |
| pDG141 | ampD in-frame deletion constructed in pDG140; contains 504-bp deletion internal to ampD | This work |
| pDG143 | ampD complementation; 680-bp fragment containing ampD under lac promoter control in pKH37 (Camr) | This work |
| Strains | ||
| MS11 | Wild-type N. gonorrhoeae (Strr) | 48 |
| DG132 | MS11 transformed with pDG132; ampG deletion mutant | This work |
| DG133 | MS11 transformed with pDG133; ampG complemented (Camr) | This work |
| DG142 | MS11 transformed with pDG141; ampD deletion mutant | This work |
| DG143 | DG142 transformed with pDG143; ampD complemented (Camr) | This work |
| DG147 | DG142 transformed with pDG017; amiC overexpression | This work |
| DG152 | DG132 transformed with pDG141; ampD ampG deletion mutant | This work |