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Validation of the O grouping and H typing multiplex PCRs using 18 reference enteric fever strains. The sample consisted of two Salmonella serovar Typhi, seven Salmonella serovar Paratyphi A, and nine Salmonella serovar Paratyphi B strains. Crude DNA extracts were tested with the O multiplex PCR (A) and H multiplex PCR (B). (C) The interpretation of the PCR products.
