TABLE 1.

List of primers used in PCRs

Region or gene namea	Primer name	Primer sequence	Reference
16S rRNA	8 f	5′-AGAGTTTGATCCTGGCTCAG-3′	20
	325 r	5′-CGCCAGAAGGCTTGGGATCATCATCTGAAG-3′	17
ITS	325 f	5′-TTCAGATGATGATCCCAAGCCTTTTGGCG-3′	17
	1100 r	5′-GAACCGACGACCCCCTGCTTGCAAAGCA-3′	17
pap31	1 f	5′-GACTTCTGTTATCGCTTTGATTT-3′	16
	688 r	5′-CACCACCAGCAAMATAAGGCAT-3′	16
rpoB	1615 f	5′-GACTTCTGTTATCGCTTTGATTT-3′	9
	2267 r	5′-CACCACCAGCAAMATAAGGCAT-3′	9
gdh1	8 f	5′-GTAAAATTATTCCAGTCTCTCCCTTT-3′
	408 f	5′-TGTCCCAATCTCATCGCAATATCACC-3′
ialB	49 f	5′-TTGAGTATTTCYTCTGYRTTTGC-3′
	530 r	5′-TGCAAAGMARTYAAACGMTTAAGWGC-3′

^agdh1 and ialB primers were designed using Bartonella quintana and B. henselae glucose-6-phosphate 1-dehydrogenase and invasion-associated protein gene sequences from complete genomes in GenBank (accession no. BX897700 and BX897699, respectively).