TABLE 1.
Lamb no. | Result(s) for sample collected at day (date) post-release onto tick-infested pasture
|
|||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
7 (9 May) | 14 (16 May) | 21 (25 May) | 28 (31 May) | 35 (7 June) | 42 (14 June) | 49 (21 June) | 56 (27 June) | 63 (5 July) | 70 (12 July) | 77 (19 July) | 84 (26 July) | 91 (2 August) | 98 (9 August) | 105 (16 August) | 112 (23 August) | |
6018 | + | A [8] (1) | A [10] (1) | B [1] | C [50] | D [3] | ||||||||||
6019 | A [22] (1) | MIX [1] | A [1] (1) | A [1] | A [1] (1) | + | F | D [1] | ||||||||
6020 | G [24] (1) | G [1] | ||||||||||||||
6021 | H | I [40] | I [3] | J [3] | J [5] | |||||||||||
6022 | E | + | + | K [3] (1) | + | F [3] (1) | F | C [1] | C [1] | L [2] | ||||||
6023 | + | M | N [2] | |||||||||||||
6024 | F [45] | + | + | I [32] | + | C [27] | ||||||||||
6025 | O [5] | P [9] | L [10] | |||||||||||||
6026 | Q | MIX [41] | O [2] | D [10] | ||||||||||||
6028 | D [69] (1) | D [1] | A [2] (1) | A [2] (1) | + | A [1] | R | P | S [6] | |||||||
6029 | H | T [1] (5) | T [3] | + | T | + | O | + | A [51] | |||||||
6031 | L [21] (1) | [1] | N [4] | L [1] | R [2] | P | ||||||||||
6032 | C [1] (6) | C [1] | C | A [2] | A [1] | C [23] | C | A | U [1] | U [2] | A [1] | |||||
6036 | E [1] | E [1] | V [57] | MIX | ||||||||||||
6045 | J [45] (1) | J | + | + | + | E | MIX [1] | |||||||||
6046 | U [1] | U | U [1] | A [2] (1) | A [1] | + | U [6] | + | + | W | O | X [13] |
Plus signs indicate blood samples that yielded only a p44 real-time PCR amplification product, whereas letters indicate samples that also yielded an msp4 PCR amplification product. Letters A to X represent the msp4 sequence type detected. Brackets indicate blood smears in which A. phagocytophilum infections were observed, and the number within the brackets is an estimate of the percentage of neutrophils infected with A. phagocytophilum; blood smears were prepared every time a blood sample was collected, but only those found to be infected are indicated in the table. Parentheses indicate that a partial 16S rRNA gene sequence was obtained for that sample, and the number within the parentheses is the sequence type encountered. Boldface indicates that the temperature of the lamb was abnormally high (>41°C) when the blood sample was collected.