FIG. 1.

α-DG expression in human airway epithelia. (A) Reverse transcription-PCR was performed using cDNA derived from primary epithelia using primers specific for α-DG or human GAPDH (20 or 30 cycles). The human-GAPDH control confirmed mRNA was isolated properly. Results are representative of findings for three different human specimens. (B) Antibodies specific for α-DG or β-DG were used to detect protein expression in a positive-control mouse myoblast cell line (C2C12) and an immortalized human airway epithelial cell line (NuLi).