FIG. 1.

Emerin- and LAP2α-deficient mouse cells. (A) The expression of emerin and LAP2α in fibroblasts derived from the single and double homozygote mice was analyzed by immunofluorescence. Staining with an emerin antibody showed the absence of the perinuclear staining pattern in all Emd^−/− cells. Staining with LAP2α antibody showed a nucleoplasmic staining pattern that was absent in Lap2α^−/− cells, although a low level of background staining was evident with this antibody (35). (B) Genotyping of primary macrophages and MEFs by RT-PCR. Total cellular RNA from mouse macrophages used in Fig. 4, below, and MEFs used in Fig. 2 and 3, below, was subjected to RT-PCR to generate cDNA. Using primers specific for emerin, LAP2α, β-actin, and the HPRT-encoding mRNA sequence, the cDNA was subjected to 30 rounds of PCR. After PCR amplification, the samples were run on 1.0% agarose to confirm genotypes. β-Actin and HPRT were amplified as controls to demonstrate the presence of RNA in the cell extracts.