FIG. 5.

HIV-1 infection in cells expressing wild-type and dominant-negative emerin. (A) 293T cells transiently transfected with pIRES-puro3, pEmerin(WT)-IRES-puro, pEmerin(m24-LEM)-IRES-puro, or pEmerin(delta-LEM)-IRES-puro were infected with HIV/VSV-G or MLV/VSV-G at similar efficiency. Cells were cotransfected with pDsRed-monomer-N1 (Clontech) at a threefold-lower molar amount as a transfection control. The percent infectivity was measured as the percentage of GFP-positive cells in the DsRed-positive population as measured by FACS. (B) Top panel: expression of wild-type emerin or mutant emerin proteins in 293T cells used in the above infections was checked using Western blotting by probing with an anti-emerin polyclonal antiserum (Santa Cruz Biotechnology, Inc.). Bottom panel: the blot was reprobed with monoclonal antibody to α-tubulin (Sigma) to confirm cell lysate loading consistency.