TABLE 3.
In vitro infectivities of AAV-DJ and wild-type vectorsa
| Cell line | Tissue or cell type | Infectivity of vector:
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| AAV-1 | AAV-2 | AAV-3 | AAV-4 | AAV-5 | AAV-6 | AAV-8 | AAV-9 | AAV-DJ | AAV-DJ/8 | ||
| Huh-7 | hu liver | 4e3 | 5e2 | 2e4 | 2e6 | 4e5 | 5e3 | 7e4 | 7e6 | 1e2 | 3e5 |
| 293 | hu kidney | 2e3 | 5e2 | 2e4 | 7e5 | 4e5 | 1e4 | 7e4 | 7e5 | 1e2 | 2e5 |
| HeLa | hu cervix | 7e4 | 2e3 | 1e5 | 2e6 | 3e4 | 2e5 | 1e6 | 2e6 | 3e2 | 1e6 |
| HepG2 | hu liver | 2e6 | 5e4 | 3e5 | 2e7 | 3e6 | 1e6 | 2e7 | ND | 4e3 | 1e7 |
| Hep1A | mu liver | 1e4 | 2e3 | 1e6 | 2e5 | 2e6 | 2e5 | 1e6 | 2e7 | 5e2 | 2e6 |
| 911 | hu retina | 6e3 | 1e3 | 9e3 | 5e5 | 7e5 | 6e3 | 1e6 | ND | 2e2 | 4e5 |
| CHO | ha ovary | 1e4 | 1e4 | 7e4 | 7e5 | 3e3 | 2e4 | 1e5 | 1e6 | 4e1 | 2e5 |
| COS | si kidney | 3e3 | 1e3 | 3e3 | 3e4 | 2e4 | 7e3 | 5e4 | 2e5 | 2e2 | 3e5 |
| MeWo | hu skin | 2e3 | 2e2 | 1e3 | 7e4 | 3e3 | 2e3 | 2e4 | 1e5 | 7e0 | 2e4 |
| NIH3T3 | mu fibroblasts | 2e5 | 2e4 | 7e5 | 7e5 | 7e6 | 2e5 | 7e6 | ND | 4e3 | 2e7 |
| A549 | hu lung | 7e4 | 1e4 | 5e4 | ND | 2e6 | 1e5 | 2e6 | 7e6 | 1e3 | 2e7 |
| HT1180 | hu fibroblasts | 5e4 | 1e4 | 1e5 | 7e6 | 3e6 | 3e4 | 2e6 | 1e7 | 3e3 | 5e6 |
| Monocytes | hu primary monocytes | 9e5 | 1e7 | ND | ND | 8e6 | 7e5 | ND | ND | 1e7 | ND |
| Immature DC | hu monocyte-derived DC | 8e5 | 2e7 | ND | ND | 9e6 | 7e5 | ND | ND | 1e7 | ND |
| Mature DC | hu monocyte-derived DC | 9e5 | 2e7 | ND | ND | 6e6 | 6e5 | ND | ND | 2e7 | ND |
Each cell line was infected with 10-fold serial dilutions of each serotype, AAV-DJ, or the mutant AAV-DJ/8 expressing a gfp reporter gene. Vector preparations were normalized to contain 2 × 109 total (vector DNA-containing) particles per ml prior to infection. Three days later, green fluorescent protein-expressing cells were counted and infectious titers were determined by taking into account the dilution factor. Numbers shown are average ratios (rounded) of total to infectious AAV particles from at least three independent titrations. Lower numbers indicate higher levels of infectivity. For each cell line, values corresponding to the most efficient AAV are underlined, while boldface indicates the lowest level of efficiency. AAV-DJ vectors showed the highest levels of infectivity on all tested cell lines. hu, human; mu, murine; ha, hamster; si, simian; DC, dendritic cells; ND, not detectable (>2 × 107).