TABLE 1.
Plating efficiency of wild-type φX174
| Strain | Plating efficiencya of protein with substitution for G61 expressed in vivo
|
|||||||
|---|---|---|---|---|---|---|---|---|
| Gb | V | D | K | P | S | A | T | |
| Wild type | 1.0 (100) | 10−2 (0.1) | <10−4 (0.3) | <10−4 (0.1) | <10−4 (0.2) | 1.0 (14) | 1.0 (5.0) | 1.0 (6.0) |
| nullD mutant | 1.0 | <10−4 | <10−4 | <10−4 | <10−4 | <10−4 | <10−4 | <10−4 |
a
The plating efficiency for wild-type φX174 is the titer on a cell line expressing a mutant external scaffolding protein/titer on a cell line expressing the wild-type external scaffolding protein. Burst sizes are given in parentheses as the percentage of the control infection, which was 128 phage/cell.
b
That is, the wild-type protein.