Replication properties. (A) One-step growth kinetics. Replication of PrV-Ka, PrV-UL36(C26S), and PrV-UL36(C26S)R was assessed after infection of RK13 cells. Average titers and standard deviations of three independent experiments are shown. (B) Determination of plaque sizes. Plaques of PrV-Ka, PrV-UL36(C26S), and PrV-UL36(C26S)R on RK13 cells were measured, and mean plaque diameters of 30 plaques, determined in at least three independent experiments, were calculated as percentages of plaque size by PrV-Ka. Standard deviations are indicated.