FIG. 4.

Replication of POKA and the ORF10-to-ORF12 cluster gene mutants in human tonsil T cells. Primary human tonsil T cells were infected by incubation with HELF cell-infected monolayers. At 48 h postinfection, T cells were collected and stained with anti-CD3 antibody and polyclonal anti-VZV human IgG and analyzed by flow cytometry. (A) T-cell flow cytometry assay. The bars represent the folds of CD3 and VZV double-positive T cells detected for T cells incubated with POKA or the mutant viruses in comparison to values for the uninfected T-cell control. The error bar indicates the standard deviation. (B) Titrations of HELF cell-infected monolayers. Virus titers were determined by an infectious focus assay using melanoma cells. Each bar represents the mean titer from three independent wells, and the error bars represent the standard deviations.