FIG. 5.

Effects of cluster gene deletions on VZV virion morphogenesis in vitro. Melanoma cells were fixed and examined by TEM at 48 h postinfection with POKA and the ORF10-to-ORF12 cluster gene mutants. The viruses tested are indicated at the left. Column A shows nucleocapsid formation in nuclei; the arrows point to the nuclear membranes. Column B shows virions in cytoplasmic vacuoles. Column C shows the structure of extracellular virions; mature virus particles with glycoprotein envelope projections are indicated by arrows. Bars, 0.5 μm (A and B) and 0.2 μm (C). N, nucleus; C, cytoplasm; V, vacuoles.