FIG. 2.

Association of BE6 with paxillin in MEFs. (A) Paxillin-null MEFs (12) were stably transduced with Flag-paxillin and or BE6. BE6 cannot be directly detected in lysates by Western blotting. (B) C127 cells transduced with either vector or wild-type BE6 were immunoprecipitated at 4°C for 1 h with either paxillin monoclonal antibody 349 (BD Biosciences) or isotype control EE monoclonal antibody as indicated. SDS-eluted samples from washed beads were Western blotted as described in Materials and Methods. Detached cells were incubated in complete medium at 37°C for 1 h prior to lysis.