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Mapping the BE6 interaction motifs of paxillin and HIC-5. (A) In vitro binding of in vitro-translated BE6 with GST fusions to LD motifs were performed as previously described (9, 30). (B) Association of HIC-5 LD1 with BE6. In vitro-translated HIC-5 and HIC-5 deleted of the first 17 residues that contain LD1 were tested for in vitro association with bacterially expressed MBP-BE6 as described previously (30).
