TABLE 1.
Mutational analysis of paxillin in transformation of MEFs by BE6
| Paxillin moleculea | Anchorage-independent colony formationb:
|
|
|---|---|---|
| Without BE6 | With BE6 | |
| Wild-type PXN | - | ++++ |
| Flag-PXN | - | ++ |
| Flag-46 kDa-PXN | - | ++++ |
| Flag-PXN-ΔLD1,2,4 | - | - |
| Flag-PXN 1-341 | - | - |
| Flag-PXN ΔLIM1 | - | - |
| Flag-PXN-ΔLIM4 | - | - |
a
The paxillin (PXN) mutants previously described (32).
b
Colony formation in agarose was determined four times as shown in Fig. 3, and average results are presented. Scale: ++++, colony formation >50% that observed by wild-type paxillin; ++, colony formation 25 to 50% that of WT paxillin in three separate assays; −, no colony formation.