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. 2008 Apr 2;82(12):5999–6008. doi: 10.1128/JVI.00203-08

FIG. 3.

FIG. 3.

Growth analysis of WT, ts, and revertant viruses. (A) Virus growth at 40°C. DBT-9 cells were infected with the indicated viruses at an MOI of 0.01 PFU/cell. Samples of virus supernatants were obtained at 1, 4, 8, 12, 16, 24, and 36 h p.i., and virus titers were determined by plaque assay at 30°C on DBT-9 cells. (B) Virus growth at 30°C. DBT-9 cells were infected with the indicated viruses at an MOI of 0.01 PFU/cell. Samples of virus supernatants were obtained at 1, 4, 8, 12, 16, 24, and 36 h p.i., and virus titers were determined by plaque assay at 30°C on DBT-9 cells. (V148A, nsp5/V148A virus; S133N/V148A, nsp5/S133N/V148A virus; H134Y/V148A, nsp5/H134Y/V148A virus).