TABLE 1.

Oligodeoxynucleotides used for the mutagenesis of MHV cDNA C fragment plasmids

Primer target	Polarity	Sequencea
nsp5/V148A	Sense	5′-AGT TAA AAC ATA TCC TGC AGA ACC GCA GGA TCC-3′
	Antisense	5′-GGA TCC TGC GGT TCT GCA GGA TAT GTT TTA ACT-3′
nsp5/S133N	Sense	5′-GTT ACG CTT CGT AGT AAC CAT ACC ATA AAG GGC-3′
	Antisense	5′-GCC CTT TAT GGT ATG GTT ACT ACG AAG CGT AAC-3′
nsp5/H134Y	Sense	5′-ACG CTT CGT AGT AGC TAT ACC ATA AAG GGC TCC-3′
	Antisense	5′-GGA GCC CTT TAT GGT ATA GCT ACT ACG AAG CGT-3′

^aUnderlined nucleotides represent nucleotides altered from the WT sequence.