TABLE 2.
Determination of nsp5 mutant virus ts phenotype
| Virus strain | Amino acid at position:
|
Titer at:a
|
EOPb | |||
|---|---|---|---|---|---|---|
| 133 | 134 | 148 | 30°C | 40°C | ||
| WT | Ser | His | Val | 9.0 × 107 | 3.0 × 108 | 3.3 |
| Alb/ts/nsp5/V148A | Ser | His | Ala | 2.8 × 108 | 8.5 × 104 | 3 × 10−4 |
| nsp5/V148A virus | Ser | His | Ala | 1.0 × 108 | 2.0 × 103 | 2 × 10−5 |
| nsp5/S133N virus | Asn | His | Val | 1.8 × 106 | 5.5 × 106 | 3.1 |
| nsp5/H134Y virus | Ser | Tyr | Val | 6.5 × 105 | 2.0 × 106 | 3.1 |
| nsp5/S133N/V148A virus | Asn | His | Ala | 2.8 × 107 | 3.8 × 107 | 1.4 |
| nsp5/H134Y/V148A virus | Ser | Tyr | Ala | 4.0 × 106 | 4.3 × 106 | 1.1 |
a
Values for titers indicated are in PFU/ml.
b
EOPs are titers at 40°C divided by titers at 30°C.