FIG. 8.
5′ caps restored stability to C24A and 2APro(−) PV mRNAs. (A and B) Cell-free translations were programmed with 50 μg/ml of uncapped (A) or 5′-capped (B) 32P-labeled PV RNA2, C24A, or 2APro(−) mRNA. Acid-precipitable 32P-labeled RNA (32P-RNA) (in cpm per μl) was measured after 0, 20, 40, 60, 120, and 240 min of incubation. (C) The amount of intact PV mRNA after 240 min of incubation was also determined by fractionation of RNA by electrophoresis in 1% agarose and quantification by phosphorimaging. The migration positions of intact PV mRNAs are indicated by arrows to the left of the gels. WT, wild type.