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. 2008 Apr 9;82(12):6017–6023. doi: 10.1128/JVI.00387-08

FIG. 3.

FIG. 3.

Results of helicase assay of LTAg mutations. Each lane in all panels contains 15 fmol dsDNA substrate. Three protein concentrations (50, 100, 200 ng protein or 0.85, 1.7, 3.4 pmol monomer) for each protein sample (labeled above the gels) were used in the assay. Lane 1, boiled dsDNA substrate; lane 2, dsDNA substrate alone. The circle labeled with an asterisk indicates radiolabeled ssDNA annealed to nonradiolabeled M13 ssDNA. The horizontal line labeled with an asterisk indicates unwound radiolabeled ssDNA.