TABLE 1.
Determination of the N-terminal sequences of the GP2 parts of AMPV, JUNV, and LASVa
| Arenavirus | Amino acid at indicated position
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Predicted N terminus of GP2
|
Determined N-terminal sequence
|
||||||||
| Major sequence
|
Minor signals
|
||||||||
| +1 | +2 | +3 | +1 | +2 | +3 | +1 | +2 | +3 | |
| LASV | G | T | F | G | T | F | |||
| JUNV | A | F | F | A | F | F | G | L | |
| AMPV | A | F | F | A | F | F | G | ||
a
The predicted N-terminal sequences of AMPV, JUNV, and LASV are as reported previously (1). The residue +1 indicates the N terminus of GP2 after proteolytic processing. The N-terminal sequences of the GP2 parts of recombinant, flag-tagged GPs of AMPV, JUNV, and LASV were determined by Edman degradation (for details, see the text). The amino acids of the major sequences correspond to the major peaks obtained in the sequencing cycle at the given position. Minor signals observed in some cases are also indicated.