FIG. 2.

(A) Replication kinetics of the viral PR mutants. Recombinant viruses containing patient-derived viral PRs with multiple amino acid substitutions were prepared. VPR1 contains an E35EE insertion and VPR3 contains an L33LL insertion. VPR2 and VPR4 are derivatives of VPR1 and VPR3, respectively, lacking the corresponding insertions. Viral replication experiments were performed with these recombinant viruses in SupT1 cells. Virus replication was monitored by p24 production in the culture supernatant. (B and C) Replication competition experiments, comparing the replication capacity of VPR1 relative to VPR2 (B) and VPR3 relative to VPR4 (C). Replication competition assays were performed in SupT1 cells in two independent experiments. At several time points during viral culture, the relative presence of both viruses in the population was determined by sequence analysis.