TABLE 1.
List of mutations and enzyme characteristics (Km, kcat, and catalytic efficiency) of PR variants analyzed in this study
| HIV-1 PR type | Mutationsa | Enzyme kineticsb
|
||
|---|---|---|---|---|
| Km (μM) | kcat (s−1) | kcat/Km (mM−1·s−1) | ||
| Wild-type | 15.1 ± 1.3 | 30 ± 1.8 | 1,990 ± 210 | |
| PR1 | L10F, I13V, G16A, K20M, V32I, E35EE, K43T, M46V, I47V, I54M, I64V, A71V, V82A | 20.4 ± 1.6 | 5.5 ± 0.2 | 271 ± 23 |
| PR2 | L10F, I13V, G16A, K20M, V32I, K43T, M46V, I47V, I54M, I64V, A71V, V82A | 9.2 ± 1.0 | 6.5 ± 0.3 | 712 ± 80 |
| PR3 | L10I, I13V, K20I, L33LL, M36I, M46I, I54V, K55R, Q58E, I62V, L63P, C67F, A71V, G73S, V82A, L90M | 23 ± 1 | 9.1 ± 0.4 | 400 ± 25 |
| PR4 | L10I, I13V, K20I, M36I, M46I, I54V, K55R, Q58E, I62V, L63P, C67F, A71V, G73S, V82A, L90M | 11.3 ± 0.7 | 8.3 ± 0.4 | 735 ± 53 |
a
Amino acid insertions are underlined.
b
Values are means ± standard deviations. Catalytic efficiency is calculated as kcat/Km.