FIG. 5.

Graphical representation of the correlation of EBNA1-binding affinity for its origin binding sites in the presence or absence of TRF2-half-binding sites with measured replicative efficiencies. The rank order of affinities of EBNA1-binding sites are placed on the x axis, from weakest to strongest (left to right); the replicative efficiencies in the short-term assay (from Table 1) and the colony formation assay (from Table 2) are placed on the y axis. A positive correlation between the affinity of EBNA1 for its origin-binding sites with both replicative efficiencies is observed in the presence of TRF2-half-binding sites but not in their absence. Derivatives of wtDS lacking the TRF2-half-binding sites were not constructed for the present study, and hence the replicative efficiencies were not determined in these assays.