TABLE 1.

Comparison of the short-term replication efficiencies of the natural and artificial origins of DNA synthesis in Raji cells

Plasmid	Origin description	Normalized replicative efficiency	SD
3487	wtDS	1	NA
3488	MCS-only	0.03	0.04
3508	2x(DS1+2)	0.15	0.06
3509	4xFRbs	0.12	0.08
3510	4xRep*bs	0.001	0.01
3511	4xSiteIIIbs	0.27	0.01
3516	S-W-W-S	0.61	0.19
3512	2x(DS1+2) + TRF2(1/2, 1/2, 1/2)	0.57	0.28
3513	4xFRbs + TRF2(1/2, 1/2, 1/2)	2.20	0.60
3514	4xRep*bs + TRF2(1/2, 1/2, 1/2)	0.28	0.09
3515	4xSiteIIIbs + TRF2(1/2, 1/2, 1/2)	0.86	0.10
3591	wtDS + TRF2(1/2, 1/2, 1)	1.7	0.65
3592	4xFRbs + TRF2(1/2, 1/2, 1)	0.73	0.03
3603	wtDS + TRF2(1, 1/2, 1)	1.77	0.92
3604	4xFRbs + TRF2(1, 1/2, 1)	0.92	0.33