FIG. 6.

Microglia from JR-CSF/hu-cycT1 mice produce increased CCL2/MCP-1 in response to LPS. JR-CSF/hu-cycT1 mice, JR-CSF mice, or control littermates (n = 3 mice/group) were injected intracranially with LPS. The mice were killed 3 days later, and paraffin sections from the brains were examined by confocal immunofluorescence microscopy after being stained with DAPI (upper left), anti-CCL2/MCP-1 antibody (lower left), and anti-GFAP antibody (upper right). A merged view also is shown (lower right). Photomicrographs (scale bar, 75 μm) of representative fields of LPS-injected control, JR-CSF, and JR-CSF/hu-cycT1 mouse brains are shown.