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. 2008 Mar 19;82(11):5161–5166. doi: 10.1128/JVI.02694-07

FIG. 3.

FIG. 3.

Recognition of NP derived from H3N2 and H5N1 influenza virus by in vitro expanded PBMC specific for influenza virus A/NL/18/94 (H3N2). The lytic activity of in vitro expanded PBMC was tested with MHC-I matched BLCL nucleofected with NP-GFP coding plasmid (NP of either influenza virus A/NL/18/94 [black circles] or A/VN/1194/04 [open circles]) or empty control GFP plasmid (gray circles). The NP-specific lytic activity was tested with PBMC of the subjects from group I (subjects 1 to 4), II (subjects 5 to 9), and III (subjects 10 to 14 and 16). E:T ratios were 0, 3.125, 6.25, 12.5, 25, and 50 for all subjects except numbers 13 and 14, for whom E:T ratios were 0, 0.3, 1, 3, 10 and 30. The lytic activities against control plasmid-transfected target cells are not visible for subjects 13 and 14 as a result of negative values for the percentages of specific lysis, which were caused by slight increases in the number of GFP-positive viable cells. Standard deviation of the means was <10%.