FIG. 10.

Localization of TGN46 in Vero cells infected with wild-type HSV-1. Vero cells were infected with HSV-1(F) at an MOI of 10 for 12 h, fixed, permeabilized, and then stained with antibody to TGN46 and gB. Anti-TGN46 antibodies were detected with AlexaFluor 488-conjugated donkey anti-sheep immunoglobulin G, and anti-gB antibodies were detected with AlexaFluor 546-conjugated goat anti-mouse immunoglobulin G. Samples were then examined by confocal microscopy. Confocal Z-sections were acquired through the entire thickness of the cells, and images of sections at the bottom and middle of the cell are shown. Single-color images were captured separately and are also shown (rows TGN46 or gB). Merged frames show simultaneous acquisition of the two colors. Insets show magnified images of assembly sites.