FIG. 9.

Visualization of recruitment of TGN markers into assembly sites formed close to the basal surfaces of HSV-1-infected Vero cells. Vero cells mock infected (A) or infected with YK608 at an MOI of 10 (B and C) were fixed at 12 h postinfection, permeabilized, and then stained with antibody to TGN46, GM130, Golgi 58K, or EEA1, which were detected with AlexaFluor 680-conjugated donkey anti-sheep immunoglobulin G or goat anti-mouse immunoglobulin G and examined by confocal microscopy. Confocal Z-sections were acquired through the entire thickness of the cells. Images of sections at the middle and differential interference contrast of the mock-infected cells are shown in panel A, and those at the bottom of the cells infected with YK608 are shown in panels B and C. (A) Mock-infected Vero cells. Localization of TGN46 (a), GM130 (c), and Golgi 58K (e) and differential interference contrast (b, d, and f) of each cell are shown. (B and C) YK608-infected Vero cells. Single-color images were captured separately, and localization of gB, VP22, VP26, or each organelle marker is shown. Frames e, k, and o show simultaneous acquisitions of the four colors. Insets show magnified images of individual assembly sites.