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. 2008 Mar 19;82(11):5417–5428. doi: 10.1128/JVI.02666-07

FIG. 4.

FIG. 4.

Infectivity of HIV-1 Env mutants. (A and C) HIV-1 viruses containing the Env MSD core mutations (A) and viruses containing truncated Env plus the MSD core mutations (C) were produced by transfecting proviral DNA into 293T cells. The p24-normalized cell culture supernatant was used to infect JC53BL indicator cells. Cells were lysed and luciferase enzyme activity was assayed after 48 h of incubation. (B and D) Single-round infectivity assay. Env-defective proviral construct pSG3Δenv was cotransfected into 293T cells with WT and mutant Env expression vectors (B) and cytoplasmic domain-truncated Envs (D). Pseudotyped viruses in the cell culture supernatant were quantitated using a p24 ELISA assay. The p24-normalized supernatant was then used to infect JC53BL indicator cells. Infectivity of MSD mutants is shown as relative luciferase activity compared to that of the WT. Results are from three independent experiments; error bars represent standard deviations from the means.