FIG. 5.

Cell surface level of Env glycoprotein and cell-cell fusion examined in the context of provirus expression. (A) 293T cells transfected with pNL4-3 proviral vectors were fixed and were labeled with Alexa 647-conjugated anti-gp120 MAb b12. Cells were then permeabilized and stained with Alexa 488-conjugated anti-p24 MAb 183-H12-5C as a transfection efficiency control. (B) The surface levels of Env glycoprotein are shown as relative mean fluorescence index (MFI) compared to that of WT. (C) Cell-cell fusion in the context of viruses. 293T cells transfected with proviral DNA were mixed with JC53BL indicator cells and cocultured for 6 h, 12 h, and 24 h before the measurement of luciferase activity. P values were calculated using Student's t test. Results are from three independent experiments; error bars represent standard deviations from the means.