FIG. 5.

Bimolecular fluorescence complementation. The graph depicts the fluorescence of purified capsids (gray bars) produced in 293TT cells by cotransfecting an L1 expression plasmid together with the L2 constructs shown to the left of the figure. Light gray bars show the fluorescence of live HeLa cells transfected with the L2 constructs shown (without any L1 plasmid). The terms “NYFP” and “CYFP” refer to N-terminal and C-terminal fragments of YFP, respectively. Fluorescence intensity was standardized to the fluorescence of L2 fused to full-length YFP (L2-YFP). The relative infectious titer of each capsid stock is given to the right of the graph. w.t., wild type.