FIG. 5.

Suppression of CyPA expression prevents infection by HCVcc/JFH-1. (A to C) The indicated stable cell lines were infected with JFH-1 for 9 to 12 days (9 days for panels A and B and up to 12 days for panel C), at which time (A) total RNA was isolated and subjected to RT-PCR detection of either HCV RNA or a cellular gene, RNA helicase A (RHA). (B) The cells were fixed with paraformaldehyde and stained with an anticore antibody. DAPI, 4′,6′-diamidino-2-phenylindole. (C) Total proteins were extracted and subjected to immunoblotting with an anti-NS3 antibody. (D) Infection of Huh-7.5/sh-A161 cells by vesicular stomatitis virus (VSV). Huh-7.5 cells with or without sh-A161 were subjected to infection by serial dilutions of VSV, ranging from 50 to 5 × 10⁴ PFU. A plaque assay was then performed to quantify infection results. (E) Inhibition of an existing infection by sh-A161. Huh-7.5 cells were first infected with HCVcc/JFH-1 for 10 days and then transduced with either sh-Luc or sh-A161 vector. One week after transduction, total protein was extracted for immunoblotting for detection of the indicated proteins.