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Relative expression levels of CyPA, CyPB, and CyPC in HCV replicon cells. (A) CyPA is expressed at a much higher level than is CyPB or CyPC. Total RNA was extracted from GS5 and RS2 cells and subjected to reverse transcription with oligo(dT) primers. The cDNA was then divided into four equal parts for the detection of CyPA, CypB, CyPC, and GAPDH mRNA with real-time PCR.
