Skip to main content
. 1999 Nov 9;96(23):13357–13362. doi: 10.1073/pnas.96.23.13357

Figure 7.

Figure 7

Electrophoretic mobility shift assay showing the effect of dexamethasone on the binding activity of nuclear extracts from EA.hy 926 cells to a double-stranded oligonucleotide containing the binding motif for transcription factor GATA of the human NOS III promoter (positions −239 to −218; 5′-GCTCCCACTTATCAGCCTCAGT-3′). Nuclear extracts were prepared from control cells (Co) or from cells incubated for 18 h with dexamethasone (10 nM to 1 μM) and were incubated with the radiolabeled oligonucleotide. Samples were analyzed on native polyacrylamide gels. Lane 1 shows radiolabeled oligonucleotide alone (oligo only). Lanes 5 and 6 (Cp1 and Cp2) show competition experiments with extracts from control (Co) cells and the addition of 100-fold excess of unlabeled oligonucleotide 5′-CACTTACAGAAAGTGATAACTCT-3′ (containing a consensus GATA binding motif), or unlabeled oligonucleotide 5′-GCTCCCACTTATCAGCCTCAGT-3′ (containing the GATA site of the human NOS III promoter), respectively. The gel shown is representative of four gels yielding the same results.