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. 2008 Mar 19;82(11):5167–5177. doi: 10.1128/JVI.00272-08

FIG. 1.

FIG. 1.

Production and molecular characterization of virus-derived siRNAs in TRV-infected plants. (A) Schematic representation of the TRV-PDS recombinant system. (B) Bleached phenotype caused by PDS silencing in TRV-infected N. benthamiana plants. (C) RNA gel blot analysis showing time course accumulation of genomic (g) and subgenomic (sg) TRV RNAs (top) and TRV siRNAs (bottom) after infection with TRV in N. benthamiana. Blots were probed with a radiolabeled DNA fragment corresponding to the 3′ UTR of TRV (top) and the 134-kDa protein-coding region of TRV1 (bottom). The positions of RNA size markers are shown on the left side of each panel. Ethidium bromide-stained RNA (prior to transfer) is shown as a loading control. dpi, days postinoculation. (D) Histogram of sizes of cloned TRV siRNAs from infected N. benthamiana and Arabidopsis. (E) Distribution of cloned sense (S) and antisense (A) TRV siRNA sequences along the TRV1 and TRV2 genomes from infected N. benthamiana and Arabidopsis plants.