TABLE 3.

Many common escape variants of Mamu-B*17 epitopes retain the ability to bind the Mamu-B*17 molecule

Epitope	Sequencea	IC50 (nM)b	Binding reduction (fold)
Vif HW8	HLEVQGYW	2.9
H1Y	YLEVQGYW	434	150
H1Q	QLEVQGYW	492	170
Nef IW9	IRYPKTFGW	3.5
I1T	TRYPKTFGW	82	23
T6M	IRYPKMFGW	94	27
Nef MW9	MHPAQTSQW	3.7
M1I	IHPAQTSQW	65	18
M1V	VHPAQTSQW	294	79
Env FW9	FHEAVQAVW	7
H2Q	FQEAVQAVW	8.6	1.2
V8I	FHEAVQAIW	13	1.9
V8F	FHEAVQAFW	14	2
V5A	FHEAAQAVW	15	2.1
H2Y	FYEAVQAVW	1,313	188

^aMutations (underlined) that result in substantial binding reductions (>100-fold) are shown in boldface.

^bRelative binding of the four Mamu-B*17-restricted peptides and common escape variants of the epitopes. IC₅₀ is the concentration of peptide needed to reduce binding of a radiolabeled reference peptide by 50%. The cutoff for physiologically relevant binding is 500 nM (55).