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. 2008 Mar 19;82(11):5153–5160. doi: 10.1128/JVI.00162-08

FIG. 2.

FIG. 2.

A16 and G9 selectively copurify with A56/K2. (A) HeLa cells were mock infected (M) or infected with VACV WR, vA56TAP, or vA28iA56TAPJ5Flag with (+) or without (−) IPTG. After 24 h, the A56 protein was isolated from the infected cell lysates by successive bindings to streptavidin and calmodulin beads. Western blotting was performed on the starting material (PreTAP) and affinity purified proteins (TAP) as described in the legend to Fig. 1. (B) BS-C-1 cells were mock infected or infected with VACV WR or vA28iA56TAPG93XFlag (with or without IPTG). After 24 h, the A56 proteins were isolated by binding to streptavidin Sepharose. Western blotting was performed on the starting material (Start) and affinity purified proteins (Streptavidin) as described in the legend to Fig. 1.