FIG. 3.

A56/K2 binds epitope-tagged A16 and G9. BS-C-1 cells were mock infected (M) or infected with VACV WR, vA28iA163XFlag (with [+] or without [−] IPTG), or vA28iG93XFlag (with or without IPTG). Infected cells were harvested after 24 h and lysed with Triton X-100. The lysate was cleared by centrifugation, and the A16 and G9 polypeptides were isolated by binding to agarose beads conjugated with Flag antibody. The eluate (Flag IP) and starting material (Start) were separated by SDS-PAGE and analyzed by Western blotting with antibodies to the viral proteins A56, K2, A28, A16, and A21 as indicated on the side. The viruses are indicated at the top of the figure.