FIG. 5.

A16 is required for G9 to bind A56/K2. Cells were mock infected (M) or infected with VACV WR or vA16iA56TAPG93XFlag (with [+] or without[−] IPTG). Infected BS-C-1 cells were harvested at 24 h and lysed with Triton X-100. Flag antibody conjugated to agarose beads was used to purify the G9 protein. The bound material (Flag IP) was eluted from the agarose beads and separated along with the starting material (Start) by SDS-PAGE and then transferred to nitrocellulose. Western blotting was performed, using antibodies to the proteins A56, K2, A16, or A28, the Flag epitope, or glyceraldehyde phosphate dehydrogenase (GAPDH), as indicated.