FIG. 6.

A16 and G9 interact in uninfected cells. (A) 293TT cells were transfected with empty vector or plasmid DNA expressing A16 with a C-terminal influenza virus HA epitope (A16-HA) or cotransfected with plasmids expressing A16-HA and G9 with a C-terminal 3XFlag tag (G9-Flag). After 48 h, the cells were lysed with Triton X-100, the postnuclear supernatant was incubated with the Flag antibody bound to beads. The starting material (Start) and the captured proteins (Flag IP) were analyzed by Western blotting with the anti-HA antibody. (B) Cells were transfected with empty vector or G9-Flag or cotransfected with A16-HA and G9-3XFlag. The lysates were incubated with anti-HA antibody bound to beads. The starting material (Start) and the captured proteins (HA IP) were analyzed by Western blotting with the anti-Flag antibody.